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    题名: An alternativemethodtoquantify2-MIBproducingcyanobacteriain rinking waterreservoirs:Methoddevelopmentand field applications
    作者: Yi-TingChiu;Hung-KaiYen;Tsair-FuhLin
    关键词: Cyanobacteria;2-methylisoborneol (2-MIB);2-MIB-synthesis gene(mibC gene);Taste and odor;Real-time PCR
    日期: 2016-11-30
    上传时间: 2016-12-22T03:19:17Z (UTC)
    摘要: 2-Methylisoborneol(2-MIB)isacommonlydetectedcyanobacterialodorantindrinkingwatersourcesin manycountries.Toprovidesafeandhigh-qualitywater,developmentofamonitoringmethodforthe detection of2-MIB-synthesis(mibC) genesisveryimportant.Inthisstudy,newprimersMIBS02F/Rin- tended specifically forthe mibC geneweredevelopedandtested.Experimentalresultsshowthatthe MIBS02F/R primersetwasabletocapture132-MIBproducingcyanobacterialstrainsgrowninthela- boratory,andtoeffectivelyamplifythetargetedDNAregionfrom172-MIB-producingcyanobacterial strains listedintheliterature.TheprimerswerefurthercoupledwithaTaqManprobetodetect2-MIB producers in29drinkingwaterreservoirs(DWRs).Theresultsshowedstatisticallysignificant correla- tions between mibC genesand2-MIBconcentrationsforthedatafromeachreservoir(R2¼0.413–0.998; po0.05), fromallreservoirsineachofthethreeislands(R2¼0.302–0.796; po0.01),andfromalldataof the threeislands(R2¼0.473–0.479; po0.01).Theresultsdemonstratethatthereal-timePCRcanbean alternativemethodtoprovideinformationtomanagersofreservoirsandwaterutilitiesfacing2-MIB- relatedincidents.
    显示于类别:[生物科技系] 期刊論文

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