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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/3275


    Title: An alternativemethodtoquantify2-MIBproducingcyanobacteriain rinking waterreservoirs:Methoddevelopmentand field applications
    Authors: Yi-TingChiu;Hung-KaiYen;Tsair-FuhLin
    Keywords: Cyanobacteria;2-methylisoborneol (2-MIB);2-MIB-synthesis gene(mibC gene);Taste and odor;Real-time PCR
    Date: 2016-11-30
    Issue Date: 2016-12-22T03:19:17Z (UTC)
    Abstract: 2-Methylisoborneol(2-MIB)isacommonlydetectedcyanobacterialodorantindrinkingwatersourcesin manycountries.Toprovidesafeandhigh-qualitywater,developmentofamonitoringmethodforthe detection of2-MIB-synthesis(mibC) genesisveryimportant.Inthisstudy,newprimersMIBS02F/Rin- tended specifically forthe mibC geneweredevelopedandtested.Experimentalresultsshowthatthe MIBS02F/R primersetwasabletocapture132-MIBproducingcyanobacterialstrainsgrowninthela- boratory,andtoeffectivelyamplifythetargetedDNAregionfrom172-MIB-producingcyanobacterial strains listedintheliterature.TheprimerswerefurthercoupledwithaTaqManprobetodetect2-MIB producers in29drinkingwaterreservoirs(DWRs).Theresultsshowedstatisticallysignificant correla- tions between mibC genesand2-MIBconcentrationsforthedatafromeachreservoir(R2¼0.413–0.998; po0.05), fromallreservoirsineachofthethreeislands(R2¼0.302–0.796; po0.01),andfromalldataof the threeislands(R2¼0.473–0.479; po0.01).Theresultsdemonstratethatthereal-timePCRcanbean alternativemethodtoprovideinformationtomanagersofreservoirsandwaterutilitiesfacing2-MIB- relatedincidents.
    Appears in Collections:[Department of Biological Science and Technology] Papers

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