Meiho University Institutional Repository:Item 987654321/4151
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 2880/3801 (76%)
造访人次 : 3878961      在线人数 : 967
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于MUIR ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.meiho.edu.tw/ir/handle/987654321/4151


    题名: Validation of 16S rRNA and Complete rpoB Gene Sequence Analysis for the Identification of Elizabethkingia Species
    作者: 楊致慧
    贡献者: 健康暨護理學院
    关键词: species identification;phylogenetic analysis;Elizabethkingia miricola cluster
    日期: 2023-09
    上传时间: 2023-09-25T08:58:28Z (UTC)
    摘要: Bacteria in the genus Elizabethkingia have emerged as a cause of life-threatening infections in humans. However, accurate species identification of these pathogens relies on molecular tech-niques. We aimed to evaluate the accuracy of 16S rRNA and complete RNA polymerase B-subunit(rpoB) gene sequences in identifying Elizabethkingia species. A total of 173 Elizabethkingia strains with whole-genome sequences in GenBank were included. The 16S rRNA gene and rpoB gene sequences from the same Elizabethkingia strains were examined. Of the 41 E. meningoseptica strains, all exhibited>99.5% 16S rRNA similarity to its type strain. Only 83% of the 99 E. anophelis strains shared >99.5% 16SrRNA gene similarity with its type strain. All strains of E. meningoseptica and E. anophelis formed a cluster distinct from the other Elizabethkingia species in the 16S rRNA and rpoB gene phylogenetic trees. The polymorphisms of 16S rRNA gene sequences are not sufficient for constructing a phylo-genetic tree to discriminate species in the E. miricola cluster (E. miricola, E. bruuniana, E. occulta, and E. ursingi). The complete rpoB gene phylogenetic tree clearly delineates all strains of Elizabethkingia species. The complete rpoB gene sequencing could be a useful complementary phylogenetic marker
    for the accurate identification of Elizabethkingia species.
    显示于类别:[生物科技系] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    ijms-24-13007 (全文).txt43KbText1检视/开启


    检视Licence

    在MUIR中所有的数据项都受到原著作权保护.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈