Direct implantation versus platelet-rich fibrin-embedded adipose-derived mesenchymal stem cells in treating rat acute myocardial infarction

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Title:	Direct implantation versus platelet-rich fibrin-embedded adipose-derived mesenchymal stem cells in treating rat acute myocardial infarction
Authors:	Cheuk-Kwan Sun;Yen-Yi Zhen;Steve Leu;Tzu-Hsien Tsai;Li-Teh Chang;Jiunn-Jye Sheue;Yung-Lung Chen;Sarah Chua;Han-Tan Chai;Hung-I Lue;Hsueh-Wen Chang;Fan-Yen Lee;Hon-Kan Yip
Keywords:	Acute myocardial infarction;Platelet-rich fibrin scaffold;Adipose-derived mesenchymal stem cell;Angiogenesis;Left ventricular remodeling
Date:	2014
Issue Date:	2014-09-16T02:12:41Z (UTC)
Abstract:	Background: This study tested whether adipose-derived mesenchymal stem cells (ADMSC) embedded in platelet-rich fibrin (PRF) scaffold is superior to direct ADMSC implantation in improving left ventricular (LV) performance and reducing LV remodeling in a rat acute myocardial infarction (AMI) model of left anterior descending coronary artery (LAD) ligation. Methods: Twenty-eight male adult Sprague Dawley rats equally divided into group 1 [sham control], group 2 (AMI only), group 3 (AMI + direct ADMSC implantation), and group 4 (AMI + PRF-embedded autologous ADMSC) were sacrificed on day 42 after AMI. Results: LV systolic and diastolic dimensions and volumes, and infarct/fibrotic areaswere highest in group 2, lowest in group 1 and significantly higher in group 3 than in group 4, whereas LV performance and LV fractional shortening exhibited a reversed pattern (p b 0.005). Protein expressions of inflammation (oxidative stress, IL-1β, MMP-9), apoptosis (mitochondrial Bax, cleaved PARP), fibrosis (Smad3, TGF-β), and pressure-overload biomarkers (BNP, MHC-β) displayed a pattern similar to that of LV dimensions, whereas anti-inflammatory (IL-10), anti-apoptotic (Bcl-2), and anti-fibrotic (Smad1/5, BMP-2) indices showed a pattern similar to that of LV performance among the four groups (all p b 0.05). Angiogenesis biomarkers at protein (CXCR4, SDF-1α, VEGF), cellular (CD31+, CXCR4+, SDF-1α+), and immunohistochemical (small vessels) levels, and cardiac stem cell markers (C-kit+, Sca-1+) in infarct myocardium were highest in group 4, lowest in group 1, and significantly higher in group 3 than in group 2 (all p b 0.005). Conclusion: PRF-embedded ADMSC is superior to direct ADMSC implantation in preserving LV function and attenuating LV remodeling.
Relation:	International Journal of Cardiology 173 (2014) 410–423
Appears in Collections:	[Department of Nursing] Papers

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