Meiho University Institutional Repository:Item 987654321/4138
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 2872/3785 (76%)
造访人次 : 3420439      在线人数 : 404
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于MUIR ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.meiho.edu.tw/ir/handle/987654321/4138


    题名: Novel Aporphine- and Proaporphine-Clerodane Hybrids Identifiedfrom the Barks of Taiwanese Polyalthia longifolia (Sonn.)Thwaites var. pendula with Strong Anti-DENV2 Activity
    作者: 林昀生
    贡献者: 健康暨護理學院
    关键词: Polyalthia longifolia var. pendula;clerodane;aporphine;proaporphine;anti-DENV2;NS2B-NS3 protease
    日期: 2023-08
    上传时间: 2023-08-30T06:05:12Z (UTC)
    摘要: Hybrid natural products produced via mixed biosynthetic pathways are unique andoften surprise one with unexpected medicinal properties in addition to their fascinating structural complexity/diversity. In view of chemical structures, hybridization is a way of diversifying natural products usually through dimerization of two similar or dissimilar subcomponents through a C-C or N-C covalent linkage. Here, we report four structurally attractive diterpene-alkaloid conjugates polyalongarins A-D (1-4), clerodane-containing aporphine and proaporphine alkaloids, the first of its kind from the barks of Taiwanese Polyalthia longifolia (Sonn.) Thwaites var. pendula. Inaddition to conventional spectroscopic analysis, single crystal X-ray crystallography was employed tode termine the chemical structures and stereo-configurations of 1. Compounds 1-4 were subsequently subjected to in vitro antiviral examination against DENV2 by evaluating the expression level of theNS2B protein in DENV2-infected Huh-7 cells. These compounds display encouraging anti-DENV2activity with superb EC50 (2.8-6.4 uM) and CCso values (50.4-200 uM). The inhibitory mechanismof 1-4 on NS2B was further explored drawing on in-silico molecular docking analysis. Based on calculated binding affinities and predicted interactions between the functional groups of 1-4 and the allosteric-site residues of the DENV2 NS2B-NS3 protease, our analysis concludes that the clerodane-
    aporphine/proaporphine-type hybrids are novel and effective DENV NS2B-NS3 protease inhibitors.
    显示于类别:[生物科技系] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    pharmaceuticals-15-01218.pdf1958KbAdobe PDF152检视/开启


    检视Licence

    在MUIR中所有的数据项都受到原著作权保护.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈