Meiho University Institutional Repository:Item 987654321/3852
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    题名: Protective Immunity against Vibrio harveyi in Grouper Induced by Single Vaccination with Poly (Lactide-co-glycolide) Microparticles Releasing Pleurocidin Peptide and Recombinant Glyceraldehyde-3-phosphate Dehydrogenase
    作者: Liu, Shang-Pin
    Chuang, Shu-Chun
    Yang, Chung-Da
    贡献者: 健康暨護理學院
    关键词: Vibrio harveyi (V. harveyi)
    single-dose vaccine
    pleurocidin (PLE)
    recombinant glyceraldehyde-3-phosphate dehydrogenase (rGAPDH)
    poly(lactide-co-glycolide) (PLG)
    PLG-encapsulated PLE plus rGAPDH microparticles (PLG-PLE/rGAPDH MPs)
    日期: 2020-01
    上传时间: 2020-06-24T05:25:10Z (UTC)
    摘要: The peptide adjuvant, pleurocidin (PLE), and the Vibrio harveyi antigen, recombinant
    glyceraldehyde-3-phosphate dehydrogenase (rGAPDH) protein, were encapsulated with poly
    (lactide-co-glycolide) (PLG) polymers in our previous study to produce PLG-encapsulated PLE
    plus rGAPDH microparticles (PLG-PLE/rGAPDH MPs) that sustained stable release of both PLE and
    rGAPDH as well as, after two-time vaccination with MPs, generated long-term protective immunity
    against V. harveyi in grouper. Stable controlled-release of PLE plus rGAPDH from PLG-PLE/rGAPDH
    MPs is an attractive feature for developing an e ective single-dose vaccine. In the present study,
    therefore, we aim to evaluate whether single administration with PLG-PLE/rGAPDH MPs in grouper
    would result in protective immunity against V. harveyi. Peritoneal vaccination of grouper with one
    dose of PLG-PLE/rGAPDH MPs raised serum titers over a long 12-week period. Moreover, twelve
    weeks after vaccination, significant lymphocyte proliferation and maximum TNF- production were
    found in grouper immunized with a single dose of PLG-PLE/rGAPDH MPs. More importantly,
    immune responses elicited by single vaccination with PLG-PLE/rGAPDH MPs protected 80% of fish
    against a lethal peritoneal challenge of the highly virulent V. harveyi (Vh MML-1). In conclusion, our
    data truly reveal the feasibility of the development of a single-dose vaccine against V. harveyi based
    on PLG-PLE/rGAPDH MPs.
    显示于类别:[生物科技系] 期刊論文

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