Meiho University Institutional Repository:Item 987654321/3727
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    题名: Monosaccharide composition influence and immunomodulatory effects of probiotic exopolysaccharides
    作者: Chen, Yo-Chia;Wu, Yu-Jen;Hu, Chun-Yi
    关键词: Probiotics;Exopolysaccharide (EPS);Monosaccharide content;Anti-inflammatory
    日期: 2019-08-20
    上传时间: 2019-08-20T03:49:30Z (UTC)
    摘要: Exopolysaccharides (EPSs) are metabolites of probiotics that have gained wide interest recently. A strain of Lactobacillus
    reuteri Mh-001with high exopolysaccharide (EPS) production abilitywas isolated, identified, andwere
    used to investigate the anti-inflammatory effects of the EPSs. Among the three unpurified EPSs, RAW246.7 murine
    macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion
    (325.32 ± 51.10 pg/ug DNA). The second lowest TNF- α secretion occurred with EPS 2 (701.12 ±
    86.108 pg/ug DNA) from Mh-002. EPSs 4, 5, and 6 were further purified from EPS 1. Cells treated with 1 ppm
    of EPS 4 had the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA). The monosaccharide components,
    EPS 4 and EPS 1, had the highest galactose content (45±2.75% and 39±2.75%, respectively). Themonosaccharide
    percentages (galactose N rhamnose N glucose) were related to the anti-inflammatory activity of the EPSs. The
    galactose content of EPSs enhanced their anti-inflammatory effects on the macrophages. These data indicate that
    EPS possesses beneficial physiological effects such as anti-inflammatory properties, and themonosaccharide content
    显示于类别:[食品營養系] 期刊論文

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