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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/3568


    Title: Rapid Method for The Gas Chromatographic QuantitativeAnalysis to Determinate Safrole in CommercialEssential Oils
    Authors: CHAN, SHENG-CHING;CHOONG, YOUK-MENG;WENG, SHUN-HSIANG
    Date: 2018-08-21
    Issue Date: 2018-08-21T06:49:23Z (UTC)
    Abstract: Safrole is a well-known carcinogenic agent that is present in camphor trees. In this study, a gas chromatographic
    method was established to quantitate the levels of safrole in essential oils using n-decyl alcohol as an internal
    standard. The method used a nonpolar column and was able to detect concentrations of safrole as low as 5 μg/ml
    in the samples. Following addition of 2–10 mg of safrole into 1 g of essential oil extracted from Stout
    Camphor wood (Cinnamomum kanehirai Hayata) or 1–10 mg of safrole into 1 g of essential oil extracted from
    Small-fl ower Camphor wood (Cinnamomum micranthum Hayat), the recovery rates of safrole were determined.
    With direct injection of samples into the gas chromatograph, the results showed that the recovery was more
    than 96.1%, with a coeffi cient of variation below 5.6%. We then analyzed 23 commercially available Stout
    Camphor and other essential oil samples and found that 21 of them contained safrole in the range of 37.65–
    355.07 mg/g. In addition, in the heavier essential oil distilled from Small-fl ower Camphor wood, the safrole
    level was up to 642.98 mg/g. Our results demonstrated that most camphor essential oils on the market have
    a carcinogenic potential due to their high safrole levels.
    Appears in Collections:[Department of Biological Science and Technology] Papers

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