Meiho University Institutional Repository:Item 987654321/3457
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    题名: Screening transferable microsatellitemarkers across genus Phalaenopsis(Orchidaceae)
    作者: Ko, Ya‑Zhu;Shih, Huei‑Chuan;Tsai, Chi‑Chu;Liao, Hsing‑Hua Ho Pei‑Chun;Chiang, Yu‑Chung
    关键词: Phalaenopsis;Microsatellites;Polymorphism;Transferability
    日期: 2017-11-17
    上传时间: 2017-11-17T08:59:40Z (UTC)
    摘要: Background: Molecular identification based on microsatellite loci is an important technology to improve the commercial
    breeding of the moth orchid. There are more than 30,000 cultivars have been enrolled at the Royal Horticultural
    Society (RHS). In this study, genomic microsatellite primer sets were developed from Phalaenopsis aphrodite
    subsp. formosana to further examine the transferability of across 21 Phalaenopsis species.
    Methods and results: Twenty-eight polymorphic microsatellite markers were obtained using the magnetic bead
    enrichment method, with high transferability of the 21 species of the genus Phalaenopsis, especially in the subgenus
    Phalaenopsis. The 28 newly developed polymorphic microsatellite markers with high polymorphism information content
    values. The best and second fit grouping (K) are inferred as two and four by the ΔK evaluation in the assignment
    test. This result indicates that these microsatellite markers are discernible to subgenus Phalaenopsis.
    Conclusions: Our results indicate that these new microsatellite markers are useful for delimiting species within
    genus Phalaenopsis. As expected, the genetic relationships between species of subgenus Phalaenopsis can be well
    distinguished based on the assignment test. These molecular markers could apply to assess the paternity of Phalaenopsis
    as well as investigating hybridization among species of genus Phalaenopsis.
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    Ya‑Zhu Ko;Huei‑Chuan Shih;Chi‑Chu Tsai;Hsing‑Hua Ho Pei‑Chun Liao;Yu‑Chung ChiangScreening transferable microsatellite markers across genus Phalaenopsis (Orchidaceae).pdf994KbAdobe PDF1检视/开启


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