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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/3390


    Title: Proteomic surveillance of putative new autoantigens in thyroid orbitopathy
    Authors: Wu, Yu-Jen;Cheng, Kai-Chun;Hung, Chun-Tzu;Cheng, Kai-Yuan;Chen, Kuo-Jen;Wu, Wen-Chuan;Suen, Jau-Ling;Chang, Cheng-Hsien
    Date: 2017-10-30
    Issue Date: 2017-10-30T07:29:34Z (UTC)
    Abstract: Aims Thyroid orbitopathy (TO) is an autoimmune
    inflammatory disorder characterised by several ocular
    manifestations. Several autoantigens have been proposed
    to be involved in the pathogenesis of TO, but the
    autoantigen system and the mechanism of TO would be
    rather complex. In this study, an immunoproteomic
    method was used to survey novel autoantigens
    expressed in the orbital fat tissue of patients with TO.
    Methods We used immunoproteomic, ELISA and
    immunohistochemical staining methods to survey novel
    autoantigens expressed in the orbital fat tissue of
    patients with TO.
    Results Six protein spots showing high reactivity with
    the serum from the patients with TO were detected as
    candidate orbital autoantigens, and two of them
    (carbonic anhydrase 1 (CA1) and alcohol dehydrogenase
    1B (ADH1B)) were further verified by ELISA and
    immunohistochemical staining. We found that CA1 and
    ADH1B could attribute target autoantigens in this
    autoimmune disease. We discovered anti-CA1 and anti-
    ADH1B antibody prevalence to be higher in patients
    with TO (68.57%/51.43%) or Graves’ disease (GD)
    (72%/48%) than in healthy controls respectively.
    Immunohistochemical staining study revealed the
    significantly enhanced expressions of CA1 and ADH1B in
    orbital fat of TO compared with that in healthy controls.
    Conclusions We found that CA1 and ADH1B could
    attribute target autoantigens in this autoimmune
    disease. The high prevalence of these autoantibodies
    against CA1 and ADH1B in patients with TO and GD
    clarifies the potential clinical role for anti-CA1 and anti-
    ADH1B antibodies as biomarkers for GD and TO.
    Appears in Collections:[Department of Biological Science and Technology] Papers

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    Yu-Jen Wu;Kai-Chun Cheng;Chun-Tzu Hung;Kai-Yuan Cheng; Kuo-Jen Chen;Wen-Chuan Wu; Jau-Ling Suen;Cheng-Hsien ChangProteomic_surveillance_of_putative_new_autoantigens_in_thyroid_orbitopathy.pdf489KbAdobe PDF1View/Open


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