Meiho University Institutional Repository:Item 987654321/3389
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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/3389


    Title: A KDEL Retrieval System for ER-Golgi Transport of
    Authors: Wu, Yu-Jen;Wang, Robert YL;Chen, Han-Shan;Chen, Chih-Jung
    Keywords: Japanese encephalitis virus;GRP78;KDEL receptor;ER-Golgi retrieval;viral particles
    Date: 2017-10-30
    Issue Date: 2017-10-30T06:54:53Z (UTC)
    Abstract: Evidence has emerged that RNA viruses utilize the host secretory pathway for processing
    and trafficking mature viral particles and for exiting the infected cells. Upon completing the complex
    assembly process, the viral particles take advantage of the cellular secretory trafficking machinery for
    their intracellular trafficking toward the Golgi organelle and budding or export of virions. In this
    study, we showed that Japanese encephalitis virus (JEV)-induced extracellular GRP78 contains no
    KDEL motif using an anti-KDEL-specific antibody. Overexpression of the KDEL-truncated GRP78
    in the GPR78 knocked down cells significantly reduced JEV infectivity, suggesting that the KDEL
    motif is required for GRP78 function in the release of JE viral particles. In addition, we demonstrated
    the KDELR protein, an ER-Golgi retrieval system component, is associated with viral envelope
    proteins and is engaged in the subcellular localization of viral particles in Golgi. More importantly,
    accumulation of intracellular virions was observed in the KDELR knocked down cells, indicating
    that the KDELR protein mediated the intracellular trafficking of JE viral particles. Altogether, we
    demonstrated that intracellular trafficking of JE assembled viral particles was mediated by the host
    ER-Golgi retrieval system prior to exit by the secretory pathway.
    Appears in Collections:[Department of Beauty Science] Papers

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