Roles of nucleic acid substratesand cofactors in the vhs protein activity of pseudorabies virus

MUIR > Graduate Institute of Health Care > Department of Beauty Science > Papers > Item 987654321/3220

Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/3220

Title:	Roles of nucleic acid substratesand cofactors in the vhs protein activity of pseudorabies virus
Authors:	Ya‑Fen Liu1, Pei‑Yun Tsai;Lin, Fong‑Yuan;Lin, Kuan‑Hsun;Hsu, Wei‑Li
Date:	2016-09-08
Issue Date:	2016-10-20T08:07:39Z (UTC)
Abstract:	Pseudorabies virus (PrV) belongs to the α-herpesvirinae of which human simplex virus (HSV) is the prototype virus. One of the hallmarks of HSV infection is shutoff of protein synthesis that is mediated by various viral proteins including vhs (virion host shutoff), which is encoded by the UL41 gene. However, the function of PrV vhs is poorly understood. Due to the low sequence similarity (39.3%) between the HSV and PrV UL41 proteins, vhs might not share the same biochemistry characteristics. The purpose of this study was to characterize the nuclease activity of the PrV vhs protein with respect to substrate specificity, its requirements in terms of cofactors, and the protein regions, as well as key amino acids, which contribute to vhs activity. Our results indicated that, similar to HSV vhs, PrV vhs is able to degrade ssRNA and mRNA. However, PrV vhs also targeted rRNA for degradation, which is novel compared to the HSV-1 vhs. Activity assays indicated that Mg2+ alone enhances RNA degradation mediated by PrV vhs, while K+ and ATP are not sufficient to induce activity. Finally, we demonstrated that each of the four highly conserved functional boxes of PrV vhs contributes to RNA degradation and that, in particular, residues 152, 169, 171, 172, 173 343, 345, 352 and 356, which are conserved among α-herpesviruses, are key amino acids needed for PrV vhs ribonuclease activity
Appears in Collections:	[Department of Beauty Science] Papers

Files in This Item:

File	Description	Size	Format
Roles+of nucleic+acid+substrates (3).pdf		2293Kb	Adobe PDF	2388	View/Open

View Licence

Loading...