Meiho University Institutional Repository:Item 987654321/2920
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    MUIR > Seminar >   >  Item 987654321/2920


    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/2920


    Title: 五爪金英誘導人類大腸癌細胞Colo201凋亡作用之研究;Tithonia diversifolia Induced Apoptosis on Colo201 Human Colorectal Cancer Cells
    Authors: 呂姿霆;黃瑞齡
    Keywords: 五爪金英;大腸癌細胞株;細胞凋亡;DNA片段化
    Date: 2015
    Issue Date: 2015-11-20T07:38:47Z (UTC)
    Abstract: 大腸癌(Colorectal cancer),又稱為結腸直腸癌,發生於大腸、直腸及闌尾處 增生居多,在西方世界裡,這是第三個最常見的癌症,並且為第二個導致死亡的 癌症,75~95%的大腸癌發病人群沒有或少見遺傳因素,主要與飲食有關,因此 被歸諸於“文明病(civilization disorder)”。五爪金英Tithonia diversifolia為菊 科植物,在民間傳統用來治療肝病,具有保肝、抗發炎及抗腫瘤活性等功效。目 前尚無五爪金英抗大腸癌的相關研究。 本研究以五爪金英Td-F2 萃取物對Colo201 大腸癌細胞進行24、48 小時之 細胞毒性試驗(MTT assay),其結果顯示24 小時IC50為2.25 μg/ml,48 小時IC50 為3.37 μg/ml,證明五爪金英Td-F2萃取物對Colo201 大腸癌細胞具毒殺作用, 具有時間與濃度依賴性,接著藉由顯微鏡觀察及DNA ladder 電泳測定,結果顯 示DNA 片段化,綜合上述結果證明五爪金英Td-F2 萃取物具誘導人類大腸癌 Colo201 凋亡,希望未來能更進一步探討相關細胞凋亡調控機制。
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