| 摘要: | Citrus madurensis Lour. (calamondin), a perennial tree in the
family Rutaceae, is a important citrus tree in Taiwan where its juice and
fruit are popular among consumers. However, juice production often
results in a considerable amount of waste, such as peels, seeds and pulp;
fortunately, the waste are rich in flavonoids, carotenoids, polyphenols,
and limonoids compounds which all have excellent bioactivities [1-
10] and the development of these compounds could create additional
value.
The human hepatitis B virus (HBV) is a global disease that is
especially prevalent in Asia and Africa. Infections could induce acute
hepatitis that could cause the infected into chronic hepatitis carriers.
Approximately one-quarter to one-third of those infected will develop
early liver cirrhosis and are at elevated risk of hepatocellular carcinoma
[11,12]. Current treatments include Interferon-α, Lamivudine,
Adefovir, Entecavir, and Telbivudine, but these drugs can only reduce
the activity or temporally inhibit the reproduction of HBV and cannot
eradicate HBV from the human body. Therefore, in recent years,
developing antivirus drugs from natural products has become an
important research topic.
Research has revealed that flavonoids in citrus demonstrated
antivirus activities [13-17], but little research has been conducted on the
anti-HBV properties of citrus. So far the only citrus anti-HBV research
consisted of isolated imperatorin purified from pomelo (C. grandis (L.)
Osbeck) peels, in which findings displayed that imperatorin possessed
excellent inhibition effect on the surface antigen and e-antigen of HBV
[18]. The purpose of this study is to investigate the anti-HBV activity
of calamondin and citrus flanonoids. We used HBV transected cell line
MS-G2 to assess that anti-HBV ability. |
