Eugenosedin-A prevents high-fat diet increased adhesionmolecules through inhibition of MAPK- and p65-mediatedNF-kB pathway in rat model

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Title:	Eugenosedin-A prevents high-fat diet increased adhesionmolecules through inhibition of MAPK- and p65-mediatedNF-kB pathway in rat model
Authors:	Lina, Hui-Li;Shenb, Kuo-Ping;Changc, Wen-Tsan;Lind, Jou-Chun;Ane, Li-Mei;Chene, Ing-Jun;Wu, Bin-Nan
Contributors:	健康暨護理學院
Date:	2012
Issue Date:	2013-11-07T07:40:28Z (UTC)
Abstract:	Objectives Previous studies have shown eugenosedin-A, a 5-HT1B/2A and a1/a2/b1- adrenergic blocker, is able to decrease cholesterol levels, hyperglycaemia and inflammation in hyperlipidaemic mice induced by high-fat diet (HFD). The aim of this study is to examine the effects of eugenosedin-A on the inhibition of adhesion molecules of platelets, the aorta and acyl-coenzymeA:cholesterol acyltransferase-1 (ACAT-1) of macrophages in a hyperlipidaemic rat model. Methods Six-week-old Sprague–Dawley rats were randomly divided into two control and treatment groups. The control rats received either a regular diet or HFD and the treatment groups were fed HFD with either 5 mg/kg eugenosedin-A or atorvastatin for a 10-week period. Key findings Compared with the two control groups, the HFD group had lower levels of high-density lipoprotein, higher concentrations of triglycerides, total cholesterol, low-density lipoprotein and insulin. The expression of adhesion molecules in platelets, aorta and monocyte-macrophage were enhanced by HFD. HFD also increased upstream proteins and their phosphorylated form in the aorta. In treatment groups, eugenosedin-A and atorvastatin improved HFD-induced hyperlipidaemia and levels of insulin. Eugenosedin-A reduced the upregulation of P-selectin, ICAM-1, ICAM-2, ICAM-3, VCAM, PECAM in platelets and inhibited E-selectin, ICAM-1, ICAM-2, ICAM-3, VCAM and PECAM protein levels in the aorta. Eugenosedin-A reduced the ACAT-1 protein expression of monocytemacrophages. The expression of PKCa, MAPKs, IKKa and p65 and their phosphorylated form were reduced in treatment groups. Conclusions Taken together, hyperlipidaemia enhances the expression of adhesion molecules and ACAT-1 protein, and eugenosedin-A ameliorates those increases. Through inhibition of MAPK- and p-65-mediated NF-kB pathway, eugenosedin-A decreases the quantity of adhesion molecules.
Relation:	Journal of Pharmacy And Pharmacology
Appears in Collections:	[Department of Food Science and Nutrition] Papers

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