Meiho University Institutional Repository:Item 987654321/2203
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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/2203


    Title: Characterization of 42 Microsatellite Markers from Poison Ivy,Toxicodendron radicans (Anacardiaceae)
    Authors: Tsai-Wen Hsu;Huei-Chuan Shih;Chia-Chi Kuo;Tzen-Yuh Chiang;Yu-Chung Chiang
    Keywords: genetic diversity;microsatellite markers;poison ivy;poison oak;population structuring;Toxicodendron
    Date: 2013
    Issue Date: 2013-10-21T02:30:49Z (UTC)
    Abstract: Poison ivy, Toxicodendron radicans, and poison oaks, T. diversilobum and T. pubescens, are perennial woody species of the Anacardiaceae and are poisonous, containing strong allergens named urushiols that cause allergic contact dermatitis. Poison ivy is a species distributed from North America to East Asia, while T. diversilobum and T. pubescens are distributed in western and eastern North America, respectively. Phylogreography and population structure of these species remain unclear. Here, we developed microsatellite markers, via constructing a magnetic enriched microsatellite library, from poison ivy. We designed 51 primer pairs, 42 of which successfully yielded products that were subsequently tested for polymorphism in poison oak, and three subspecies of poison ivy. Among the 42 loci, 38 are polymorphic, while 4 are monomorphic. The number of alleles and the expected heterozygosity ranged from 1 to 12 and from 0.10 to 0.87, respectively, in poison ivy, while varied from 2 to 8 and, from 0.26 to 0.83, respectively in poison oak. Genetic analysis revealed distinct differentiation between poison ivy and poison oak, whereas slight genetic differentiation was detected among three subspecies of poison ivy. These highly polymorphic microsatellite fingerprints enable biologists to explore the population genetics, phylogeography, and speciation in Toxicodendron.
    Relation: Int. J. Mol. Sci. 2013, 14, 20414-20426
    Appears in Collections:[護理系] 期刊論文

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