Meiho University Institutional Repository:Item 987654321/1722
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    题名: Detection and quantification of major toxigenic Microcystis genotypes in Moo-Tan reservoir and associated water treatment plant
    作者: Hung-Kai Yen;Tsair-Fuh Lin;I-Cheng Tseng
    贡献者: 健康暨護理學院
    日期: 2012-01
    上传时间: 2012-09-06T02:23:55Z (UTC)
    摘要: Two molecular methods, denaturing gradient gel electrophoresis (DGGE) and quantitative real-time
    polymerase chain reaction (qPCR) with the Universal ProbeLibrary (UPL) probe, were developed and
    used for the characterization and quantification of several microcystin producers in Moo-Tan Reservoir
    (MTR), Taiwan and its associated water treatment plant (Shih-Men Water Treatment Plant, SMWTP).
    Internal transcribed spacer (ITS) sequence, a highly diversified region between the 16S rRNA and 23S
    rRNA genes, was used to further identify the isolated strains from MTR and also used in DGGE for the
    detection of the specific DNA fragments and biomarkers for 11 strains observed in MTR. These ITSDGGE
    biomarkers were successfully applied to monitor the community changes of potential toxigenic
    Microcystis sp. over a period of five years. Two highly specific primers were combined with UPL probes
    to measure microcystins synthesis gene (mcyB) and phycocyanin intergenic spacer region (cpcB)
    concentrations in water samples. The copy concentrations of UPL-mcyB and UPL-cpcB correlated well
    with MC-RR concentrations/water temperature and Microcystis sp. cell numbers in the water samples,
    respectively. For SMWTP, toxin concentrations were low, but the DGGE bands clearly demonstrated
    the presence of potential microcystin producers in both water treatment plants and finished water
    samples. It was demonstrated that toxigenic Microcystis sp. may penetrate through the treatment
    processes and pose a potential risk to human health in the drinking water systems.
    關聯: Journal of Environmental Monitoring
    显示于类别:[生物科技系] 期刊論文

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