Microsatellite primers in the native perennial cycad Cycas taitungensis (Cycadaceae)

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Title:	Microsatellite primers in the native perennial cycad Cycas taitungensis (Cycadaceae)
Authors:	Li-Ping Ju;Chia-Chi Kuo;Yi-Shan Chao;Yu-Pin Cheng;Xun Gong;Yu-Chung Chiang
Contributors:	Botanical Garden Division, Taiwan Forestry Research Institute, Taipei 100, Taiwan;Department of Nursing, Meiho University, Pingtung 912, Taiwan;Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424 Taiwan
Date:	2010-04
Issue Date:	2011-09-28
Abstract:	• Premise of the study: Microsatellite primers were developed for the native perennial cycad Cycas taitungensis to evaluate the genetic variation of this endangered insular species. •Methods and Results: Using a magnetic bead enrichment method and EST data, 16 primer sets were developed and identified for the native Taiwan cycad C. taitungensis. The primers amplified dinucleotide, trinucleotide, and complex repeats with 1–9 alleles per locus. Most primers also amplified DNA from C. revoluta and C. debaoensis. •Conclusions: These results indicate the utility of primers for future studies of the genetic structure of C. taitungensis. In addition, the primers are useful for further phylogeographic studies between C. taitungensis and C. revoluta, which is a closely related species.
Relation:	American Journal of Botany April 2011 vol. 98 no. 4 e84-e86
Appears in Collections:	[Department of Nursing] Papers

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